Combined paediatric liver-kidney transplantation: Analysis of our experience and literature review
1 Department of Surgery, University of the Witwatersrand, Johannesburg, South Africa
2 Transplant Division, Wits Donald Gordon Medical Centre, University of the Witwatersrand, Johannesburg, South Africa
Department of Paediatric Surgery, Chris Hani
Baragwanath Academic Hospital, University of the
Witwatersrand, Johannesburg, South Africa
Background. Renal insufficiency is increasingly common in end-stage liver disease and allocation of livers to this category of patient has escalated. The frequency of combined liver-kidney transplantation (CLKT) has consequently increased. Indications for CLKT in children differ from those for adults and typically include rare congenital conditions; subsequently limited numbers of this procedure have been performed in paediatric patients worldwide. Scant literature exists on the subject.
Methods. Subsequent to institutional approval, a retrospective chart analysis of all paediatric CLKTs performed at the Transplant Unit, Wits Donald Gordon Medical Centre, University of the Witwatersrand, Johannesburg, South Africa between January 2005 and July 2013 was conducted.
Results. Defining children as younger than 18 years of age, 43 patients had received a liver transplant since 2005, of whom 8 received a CLKT. Indications included autosomal recessive polycystic kidney disease (n=3), primary hyperoxaluria type 1 (n=4) and heterozygous factor H deficiency with atypical haemolytic uraemic syndrome (n=1). Graft combinations included whole liver and one kidney (n=5), whole liver and two kidneys (n=1) and left lateral liver segment and one kidney (n=2), all from deceased donors. Patient age ranged from 4 to 17 years (median 9) and included 4 females and 4 males. Weight ranged from 13 to 42 kg (median 22.5). We describe one in-hospital mortality. The remaining 7 patients were long-term survivors with a survival range from 6 to 65 months.
Conclusions. Although rarely indicated
in children, CLKT is an effective treatment option,
appropriately utilising a scarce resource and
significantly improving quality of life in the
Med J 2013;103(12):925-929.
The Transplant Unit, Wits Donald Gordon Medical Centre, University of the Witwatersrand, Johannesburg, South Africa has performed 43 paediatric liver transplants to date. Of these, 8 were combined liver-kidney transplants. Since the first successful kidney and liver transplants in the 1950s and 1960s respectively, both patient and graft survival have improved dramatically.1 The first successful combined liver-kidney transplantation (CLKT) was subsequently performed by Margreiter in Austria in 1984.2 Renal insufficiency, secondary to a variety of causes, is a common problem in patients suffering from end-stage liver disease, particularly in adults.3 Renal impairment in liver transplant patients amplifies the risk for both postoperative chronic kidney disease (CKD) and procedure-related mortality. With the introduction of the Model for End-Stage Liver Disease (MELD) score for liver transplantation the allocation of organs to patients with renal insufficiency has increased.1-3 Consequently, the number of CLKTs in adults has shown a considerable increase over the last few years. In children, the most common causes for CLKT are congenital diseases affecting both liver and kidney, such as primary hyperoxaluria type 1 (PH1) and autosomal recessive polycystic kidney disease (ARPKD). 2 These diseases have a low incidence and consequently CLKT in children remains a therapy performed in a very limited number of cases with little data available in the literature.1
All paediatric patients undergoing CLKT were
identified retrospectively. Eight transplants were
performed in 8 children (<18 years of age on the day
of transplant) since 2005. Recipient characteristics are
listed in Table 1.
Pertinent intra- and postoperative events were
documented, as were any postoperative complications
(particularly those related to vascular, biliary or
urinary reconstruction) and graft and patient survival
were analysed. Finally, a retrospective,
English-language PubMed search was conducted in January
2013 for all published papers relating to CLKT.
Eight CLKTs were performed, none of the children having received previous transplants. Indications included congenital hepatic fibrosis associated with polycystic kidney disease (PKD) (n=3), PH1 (n=4) and heterozygous factor H deficiency with atypical haemolytic uraemic syndrome (aHUS) (n=1). Patients ranged in age from 4 to 17 years (median 9) and included 4 females and 4 males. Weight ranged from 13 to 42 kg (median 22.5). Waiting times on the active transplant list ranged from 49 to 1 383 days (median 270). The range of post-transplant follow-up was 6 - 65 months.
Graft combinations were as follows: whole liver and one kidney (n=5); whole liver and two kidneys (n=1) and left lateral segment of liver and one kidney (n=2). Median anaesthetic time was 7 hrs and blood loss ranged from 80 to 4 970 ml (median 1 831). Median blood volume transfused was 900 ml of leukocyte-depleted packed red blood cells and other blood products, and 450 ml of cell-saved whole blood. Blood group crossmatch was identical in 7 patients and compatible in 1 patient.
Induction immunosuppression consisted of daclizumab (Zenapax) (weight <40 kg: 10 mg intravenous injection (IVI) on days 1 and 4; weight >40 kg: 20 mg IVI on days 1 and 4) and methylprednisolone (Solu-Medrol) (600 mg/m2 to a maximum of 500 mg). Postoperative maintenance immunosuppression consisted of mycophenolate mofetil (CellCept), tacrolimus (Prograf) and prednisone. In one case, everolimus was used after conversion from the calcineurin inhibitor. With respect to preoperative dialysis, 6 patients received haemodialysis, 1 peritoneal dialysis, and 1 was transplanted pre-emptively. The child with aHUS received polyvalent human normal immunoglobulin (Polygam) and plasma exchange pre-transplant, as well as for 10 days post-transplant. Eculizumab was not used as it was not available in South Africa at the time. No patients required postoperative dialysis for delayed graft function. All patients with hyperoxaluria received 3 months of postoperative haemodialysis to decrease systemic oxalate load. This patient group presented pre-transplant with manifestations of systemic oxalosis, particularly dense nephrocalcinosis. One patient underwent sequential nephrectomies, the first pre- and the second post-CLKT, both for severe nephrocalcinosis and recurrent urinary tract infections. A second patient is currently being considered for bilateral nephrectomy for the same reason. Native kidneys in the remaining patients remained asymptomatic and were left in situ.
There was one mortality in this series in a patient with ARPKD. The remaining 7 patients were dialysis free, with excellent quality of life subjectively, and a mean follow-up of 2.17 years. The mortality was related to surgical complications of hepatic artery and portal vein thrombosis peri-operatively. Although the patient was re-listed, a second liver did not become available and the patient died of acute hepatic failure. In the patient who received the two kidneys as an en bloc graft from a 2-year-old donor, one kidney infarcted secondary to renal artery thrombosis, although this had no impact on renal function. Another patient developed a wound haematoma, which resolved with conservative management. The last patient, who received a reduced left lateral segment graft and single kidney, developed abdominal compartment syndrome that required urgent decompression and closure with a large mesh.
Three of the patients
presented with mild episodes of rejection. The first had a
biochemically diagnosed episode of mild liver rejection on
day 15 post transplant. This was successfully managed with
3 doses of methylprednisolone without biopsy. The second
patient presented with a histologically confirmed episode
of liver rejection two years post transplant. This also
settled completely after increasing the tacrolimus dose
and adding methylprednisolone. The third patient, with
aHUS, was transferred to another centre for long-term
follow-up. Presenting with a clinically suggestive episode
of mild liver rejection, despite a normal liver biopsy,
the patient was successfully treated with
methylprednisolone and increased dose of tacrolimus.
Currently all 7 patients have excellent hepatic and renal
function. We could not conclusively define any
documentable immunological protection to the renal graft
in this small patient cohort.
Technical details of the CLKT
The specific surgical techniques for organ procurement,
graft preparation and the respective implant procedures
are well described. All kidneys were placed
extra-peritoneally via a right-sided Rutherford-Morrison
incision. We did not encounter the theoretical risk of
skin bridge necrosis at the lateral aspects of the right
subcostal and Rutherford-Morrison incisions where the two
approximate. The right-sided approach allows excellent
access to the distal aorta and inferior vena cava, which
may be required to establish adequate arterial inflow and
venous outflow, respectively. This is especially important
when a large donor kidney is implanted into a small child
(weighing <20 kg), when up to 30% of the
circulating blood volume is taken up by the transplanted
kidney on release of clamps. Inadequate arterial inflow
will compromise perfusion, causing subsequent necrosis of
the graft. In this situation arterial inflow to the donor
kidney is established directly off the aorta, below the
origin of the inferior mesenteric artery. This approach
was utilised in 3/8 renal grafts.
paediatric CLKTs extracted from the PubMed database are
shown in Table 2. While these total 101
cases, 3 568 cases have been
reported from 1984 to the present. The United Network
for Organ Sharing (UNOS), however, reports that 231
CKLTs have been performed in children in 41 transplant
centres.5 The median number of
transplants per centre was 3 (average 6). The majority
of transplants were for PH1 (n=52, 48.6%), ARPKD (n=20, 18.5%) and atypical
factor H deficiency (n=8, 7.4%).
Immunosuppression varied significantly from tacrolimus
or cyclosporin A monotherapy to multiple drug
combinations and even quadruple therapy with
antithymoglobulin, cyclosporin A,
methylprednisolone and azathioprine. When reported,
complications were predominantly sepsis related (n=6, 5.94%), and invariably
resulted in mortality. The relative infrequency of CLKT
in children is as a result of limited indications, which
include rare conditions with low incidences that have
not changed over the years.2 This leaves a challenging
problem in determining the optimal indications for CLKT.
The indications for CLKT in our patient cohort are
listed in Table 1. It is evident, even
with the small numbers that we are reporting on, that
our indications and numbers mirror those of the world
literature. We will discuss the three most common
indications for CLKT, namely PH1, ARPKD with congenital
hepatic fibrosis and heterozygous factor H deficiency
PH1 is an autosomal recessive metabolic condition caused by a congenital deficiency of alanine-glyoxylate aminotransferase (AGT), a hepatic peroxisomal enzyme. Patients with this deficiency metabolise glyoxylate to oxalate and glycolic acid instead of glycine.6 The kidneys are subsequently burdened with eliminating the large oxalate load, which results in deposition of calcium-oxalate crystals in the kidney and progression to chronic renal failure. The condition is often more severe in infants, with end-stage renal disease (ESRD) presenting before 2 years of age. By 15 years of age 50% of patients will have developed ESRD.1 , 3 , 7 As the glomerular filtration rate declines, oxalate is deposited systemically into bone and soft tissues including the retina, blood vessels, nerves and heart.6 If the condition is diagnosed early, and before significant renal failure is established, isolated liver transplantation (iLT) is an acceptable option, with excellent survival.7 However, this only addresses the issue of oxalate formation and does not resolve the problem of an already high burden of oxalate, which would force the kidneys to eliminate the remaining stores. This can contribute to progressive CKD requiring a renal transplant at a later stage. The converse is, however, not acceptable – graft survival after isolated kidney transplantation (iKT) for PH1 approximates 18% at 3 years, based on data from the European Dialysis and Transplant Association registry.8 This obviously occurs due to progressive nephrocalcinosis and urolithiasis and early graft failure, as hepatic AGT is still deficient, and oxalate over-production continues.
CLKT addresses both the primary metabolic insult, namely hepatic AGT deficiency, and the consequent end-organ damage secondary to oxalosis, i.e. chronic renal failure. Reported outcomes have been varied. The largest series of patients is from the European PH1 Transplant Registry; 127 transplants were performed in 117 patients between 1984 and 2005, 99 CLKTs, 22 iLTs and 6 patients underwent iLT followed by iKT. The 10-year survival was 69% for all groups.9 Factors which were clearly shown to affect outcome and improve survival were the overall clinical condition of the patient at the time of transplantation, earlier diagnosis and aggressive pre-transplantation dialysis.1 , 2 , 4 , 9 Of interest is that, despite a new liver, >50% of renal graft failures following CLKT were due to oxalate deposition in the new kidney and that hyperoxaluria was detectable up to 45 months post transplantation. This oxalate burden should not be underestimated.2 , 10 One of our patients required both haemo- and peritoneal dialysis preoperatively. Our current protocol is to perform haemodialysis 4 - 5 times per week pre-transplant and for 3 months post transplantation to decrease the oxalate load and to mitigate any damage to the renal allograft by nephrocalcinosis or urolithiasis. Consideration should also be given to the shortage of deceased donor organs and the resulting pressure to other patients on the waiting list. Thus, alternatives that should be considered once the decision to list has been taken include the feasibility of related living kidney donation as and when a deceased donor liver becomes available, as well as consideration of both related living kidney and liver transplantation simultaneously.1 , 11
ARPKD has an incidence of 1/20 000 live births and demonstrates a variable clinical progression.2 , 12 The most severely affected children present with ESRD, either in the perinatal period or during early infancy. Liver involvement is a result of congenital hepatic fibrosis or Caroli disease, which may progress to portal hypertension, hypersplenism with resultant thrombocytopenia and recurrent ascending cholangitis (Caroli syndrome).6 , 13 As the liver disease is chronic and may not necessarily progress to failure, iKT has been the standard intervention for ESRD secondary to ARPKD with minimal liver disease. Reported data on iKT show patient survival to be 89% and death censored graft survival at 100% at 5 years.14 However, Khan et al.15 reported on 14 patients who had received iKT in the presence of hepatic disease; 4/5 deaths in this series were due to complications from hepatic disease (systemic sepsis secondary to cholangitis) and 56% of the remaining survivors had complications related to congenital hepatic fibrosis at a mean of 6.3 years post transplantation. Davis et al.13 published a retrospective study of 203 patients who received iKT for similar indications. Of particular interest in this study is the 64% of sepsis-related deaths. While sepsis is acknowledged as a common cause of death among the paediatric group of kidney transplant recipients, the North American Pediatric Renal Trials and Collaborative Studies registry report this at 28.9%. 6 , 16 Although not proven, this may hypothetically suggest an increased rate of cholangitis related to congenital hepatic fibrosis, and the fact that these patients already have a heavily colonised biliary tree. This situation is possibly accentuated by subsequent immunosuppression.7 , 13 While liver disease is always present in these patients, it does not always warrant transplantation and iKT is often all that is required. When there is liver disease causing progressive and severe complications, CLKT is advocated. Although limited, outcome data following both adult and paediatric CLKT are favourable in the literature. CLKT should be considered when ESRD (as a result of ARPKD) and severe congenital hepatic fibrosis or hepatic failure are present. On occasion, liver disease secondary to the congenital hepatic fibrosis dominates the clinical picture. However, we are not aware of any cases of iLT in the literature and CLKT is recommended currently in this scenario.8 , 11
Heterozygous factor H deficiency with aHUS is a very unusual indication for CLKT, with only 7 cases described in the literature to date. Factor H is produced in the liver and regulates the activation of the complement cascade via the alternative pathway. Qualitative or quantitative deficiencies in factor H can result in the uninhibited deposition of complement, destruction of microvasculature and phenotype of HUS. There are >60 heterozygous factor H mutations that are responsible for ~30% of recurrent cases of HUS. They carry a poor prognosis, resulting in ESRD or death in 50% of patients. 3 , 9 , 17 , 18 Outcomes following iKT are equally poor, with recurrences in the range of 50 - 100%.3 , 17 CLKT addresses both the qualitative and quantitative factor H issues and restores renal function. Recently, eculizumab has been registered for the management of aHUS. Eculizumab is a recombinant, humanised monoclonal antibody targeting the complement protein C5. It protects red blood cells from chronic intravascular haemolysis by preventing generation of the C5b9 complex, thus inhibiting activation of terminal complement, which is responsible for cell lysis. The current recommendation is to perform a kidney transplant combined with lifelong eculizumab therapy.19 Unfortunately, the lifelong cost of this drug is currently prohibitively high. The alternative is CLKT, which is a more aggressive treatment option and is potentially associated with greater morbidity and mortality due to the combined organ transplant. To reduce this risk, an option is to use one dose of eculizumab peri-operatively in CLKTs to reduce the complications related to uninhibited complement activation. A second dose can be held in reserve if necessary. Where antifactor H antibodies exist a CKLT may need to be combined with lifelong plasma exchange and therapy aimed at reducing antibody levels. Our patient did not have antifactor H antibodies.
At the time of writing, CLKT has been reported in 7 patients (including our own case) with factor H-associated HUS. Most recently, Khan et al. 15 reported one case that was uncomplicated, apart from a short period of delayed renal graft function of the kidney. Saland et al.3 also published a single case with 100% dual graft survival at 2 years. Our case was equally uncomplicated, with dual graft survival >2 years post transplant. Plasma exchange has been suggested in the immediate pre-transplant period, or intraoperatively, as it restores normal factor H function and inhibits unregulated complement deposition until the new liver has had time to normalise factor H levels and function. Also, because split liver grafts are more susceptible to poor perfusion and the resultant increased complement activation, it is suggested by some units that whole liver grafts be used.18 It should be stated that this is not the practice of our unit and we would consider the use of split grafts without reservation. Although there are only a limited number of documented cases in addition to our own single case experience, it has been demonstrated that, in light of these recent successes, as well as the poor outcomes of alternative therapy, CLKT should be considered for this subgroup of patients.
Since CLKTs are now more commonly performed, there has been debate as to whether the liver allograft offers ‘immunoprotection’ to the kidney allograft. In 2003, a review of the UNOS database illustrated a lower 6-month cumulative acute renal rejection rate for patients receiving CLKT than iKT (21.5% v. 30.1%, respectively). When reviewing single-centre experiences, the results appear even more promising; however, the small numbers reported in these studies should caution their interpretation. Grewal et al. 20 reported a 42% acute renal allograft rejection rate but only 2/5 cases were proven by biopsy. Rogers et al. 21 showed a 25% acute renal allograft rejection rate v. a 86% rejection rate in the kidney after liver transplant cohort. More recently, de la Cerda et al.22 conducted a single-centre, retrospective, case-control study that looked at 10 children who had CLKT and survived to 6 months, comparing them to a control group of 20 kidney-only transplants matched for age, era and immunosuppression. In the CLKT group, only 1 acute renal allograft rejection was reported at 7 years secondary to non-compliance of medication. In the same period, the kidney-only group had 16 acute rejection episodes. There was also no hepatic rejection in the CLKT group. The study was, however, too small to uncover a significant difference in immunologically mediated kidney allograft failure. The same authors therefore looked at UNOS paediatric data, analysing 111 CLKT and 3 798 kidney-only transplants between 1995 and 2005. They found that, although renal graft loss in the first 6 months was higher in the CLKT than the kidney-only group (20.1% v. 5.9%, respectively), death-censored kidney allograft survival at 5 years was significantly better in the CLKT group and that function did not continue to deteriorate as it did in the kidney-only group.22
When reviewing the
literature there are a few reported cases where patients
required re-liver transplant after CLKT for graft
non-function or rejection. In one case, a patient
received two subsequent liver transplants following the
initial CLKT.23 Although that patient did
not ultimately survive, the feasibility of subsequent
transplantation does exist, dependent obviously on the
availability of organs. This does validate our
suggestion that our single mortality might have survived
had another organ been available and, at the same time,
raises the question of utilising a scarce resource.
Our centre demonstrates equivalent results in terms of
graft survival, morbidity and mortality compared with
reported data in the international literature. In the
paediatric age group, the most common indications for CLKT
are metabolic diseases, which affect either the kidney
alone with or without liver dysfunction or disorders
affecting both organs simultaneously. CLKT has good
results in select groups of patients and long-term
survival approaches that of liver transplantation alone.
Outcomes are optimised by early evaluation and listing,
before patients manifest systemic complications and
develop end-stage disease. It is our strong view that all
options should be utilised in transplanting these
patients, including deceased donor split livers, RLD
kidneys with deceased donor liver, and the RLD option for
both liver and kidney. In patients with PH1,
nephrocalcinosis and systemic oxalosis can be problematic
post transplant and 3 months dialysis post CLKT to clear
systemic oxalic acid is recommended. Irreversible renal
dysfunction should not exclude children with severe liver
disease from consideration for liver transplantation if it
is performed with a simultaneous kidney transplant.
Factors that negatively affect the outcome of iLT (UNOS
status and re-transplantation) must be considered in
determining the suitability of any given candidate for
CLKT but ultimately ESRD and poor quality of life need to
be offset against the potential excellent long-term
benefits of a timeous CLKT. Similarly, patients with ARPKD
demonstrate excellent graft and patient survival.
Regarding mortality occurring in ARPKD, 64 - 80% can be
attributed to systemic sepsis, likely a consequence of
cholangitis subsequent to their liver disease. As surgical
mortality in paediatric liver transplant recipients has
been shown to be <10% at one year, CLKT is a viable
option to decrease overall mortality in patients with
recurrent cholangitis or complications of portal
hypertension. There is suggestive clinical evidence that
CLKT offers a degree of immunological protection to the
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3. Saland JM, Emre SH, Shneider BL, et al. Favorable long-term outcome after liver-kidney transplant for recurrent hemolytic uremic syndrome associated with a factor H mutation. Am J Transplant 2006;6(8):1948-1952. [http://dx.doi.org/10.1111/j.1600-6143.2006.01375.x]
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10. Latta K, Jamieson NV, Scheinman JI, et al. Selection of transplantation procedures and perioperative management in primary hyperoxaluria type 1. Nephrol Dial Transplant 1995;10(Suppl 8):53-57. [http://dx.doi.org/10.1093/ndt/10.supp8.53]
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15. Khan K, Schwarzenberg SJ, Sharp HL. Morbidity from congenital hepatic fibrosis after renal transplantation for autosomal recessive polycystic kidney disease. Am J Transplant 2002 [http://dx.doi.org/10.1034/j.1600-6143.2002.20412.x]
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17. Remuzzi G, Ruggenenti P, Colledan M, et al. Hemolytic uremic syndrome: A fatal outcome after kidney and liver transplantation performed to correct factor H gene mutation. Am J Transplant 2005;5(5):1146-1150. [http://dx.doi.org/10.1111/j.1600-6143.2005.00783.x]
18. Jalanko H, Peltonen S, Koskinen A, et al. Successful liver-kidney transplantation in two children with aHUS caused by a mutation in complement factor H. Am J Transplant 2008;8(1):216-221. [http://dx.doi.org/10.1111/j.1600-6143.2007.02029.x]
19. Connock M, Wang D, Fry-Smith A. Prevalence and Prognosis of Paroxysmal Nocturnal Haemoglobinuria and the Clinical and Cost-effectiveness of Eculizumab. Department of Public Health and Epidemiology. West Midlands Health Technology Assessment Collaboration Report; 69. Birmingham: University of Birmingham, 2008.
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23. Chava SP, Singh B, Stangou A, et al. Simultaneous combined liver and kidney transplantation: A single center experience. Clin Transplant 2009;24(3):E62-E68. [http://dx.doi.org/10.1111/j.1399-0012.2010.01168.x]
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27. Loreno M, Boccagni P, Rigotti P, Naccarato R, Burra P. Combined liver-kidney transplantation in a 15-year-old boy with alpha1-antitrypsin deficiency. J Hepatol 2002;36(4):565-568. [http://dx.doi.org/10.1016%2FS0168-8278%2802%2900012-0]
28. Millan MT, Berquist WE, So SK, et al. One hundred percent patient and kidney allograft survival with simultaneous liver and kidney transplantation in infants with primary hyperoxaluria: A single-center experience. Transplantation 2003;76(10):1458-1463. [http://dx.doi.org/10.1097/01.TP.0000084203.76110.AC]
29. Nagarajan S, Enns GM, Millan MT, Winter S, Sarwal MM. Management of methylmalonic acidaemia by combined liver-kidney transplantation. J Inherit Metab Dis 2005;28(4):517-524. [http://dx.doi.org/10.1007/s10545-005-0517-8]
30. Sakamoto S, Kasahara M, Fukuda A, et al. Pediatric liver-kidney transplantation for hepatorenal fibrocystic disease from a living donor. Pediatr Transplant 2012;16(1):99-102. [http://dx.doi.org/10.1111%2Fj.1399-3046.2011.01609.x]
7 October 2013.
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