Original articles

Antioxidant and oxidative stress status in type 2 diabetes and diabetic foot ulcer

Elizabeth Bosede Bolajoko, KenseseE Sontin Mossanda, Francis Adeniyi, Olubayo Akinosun, Adesoji Fasanmade, Mpho Moropane

Abstract


ABSTRACT
Objectives: Oxidative stress (OS) has been implicated in the aetiology and progression of diabetic complications including diabetic foot ulcer. In the present study, the levels of lipid peroxides (LPO), a marker of lipid peroxidation and 8-hydroxy-2’-deoxyguanosine (8-OHdG), a marker of DNA damage as well as the enzymatic antioxidant activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in type 2 diabetes mellitus and diabetic foot ulcer subjects were assessed and compared with apparently healthy normal subjects in order to understand the involvement of OS in diabetic foot ulcer subject.
Design: The above-mentioned OS markers were measured in 50 subjects for each of the following groups: type 2 diabetes mellitus (DM), diabetic foot ulcer (DF) and non diabetic control (NC).
Results: Significant elevated values of LPO and 8-OHdG were found in DM subjects: 39.86% and 45.53% increase respectively compared to the NC subjects. This increase in both parameters was greater for DF subjects: 80.23% and 53.91% respectively. SOD activities were significantly reduced in DM (14.82%) and DF (4.09%) subjects in contrast with elevated activities of GPx observed in DM (21.87%) and DF (20.94%) subjects. In this study, positive correlations between glycated hemoglobin/fasting plasma glucose (HbA1c/FPG) and the following OS parameters: LPO, 8-OHdG and GPx were observed. However, correlation between the above glycemic components and SOD was found to be negative.
Conclusion: Increased oxidation subsequent to diabetic conditions induces an overexpression of GPx activity suggesting a compensatory mechanism by the body to prevent further tissue damage in these subjects.

Authors' affiliations

Elizabeth Bosede Bolajoko, University of Limpopo, Medunsa Campus

KenseseE Sontin Mossanda, University of Limpopo, Medunsa Campus

Francis Adeniyi, University of Ibadan

Olubayo Akinosun, University Of Ibadan

Adesoji Fasanmade, University of Ibadan

Mpho Moropane, University of Limpopo, Medunsa Campus

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Keywords

Diabetic foot Ulcer; Type 2 diabetes mellitus; Antioxidant; Oxidative stress

Cite this article

South African Medical Journal 2008;98(8):614.

Article History

Date submitted: 2008-03-06
Date published: 2008-08-04

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